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. 2008 Aug 13;36(16):5391–5404. doi: 10.1093/nar/gkn522

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — miR-16 and miR-424 triggered G1 arrest partially by regulating CCND1. (A) The effectiveness of miR-16, miR-424 and siRNA against CCND1 on CCND1 and pRb was analyzed by western blot, with mock and control Luc-siRNA transfected A549 cells as controls. Antibody against β-actin was used as a loading control. (B) miR-16 and miR-424 triggered the accumulation of cells at G0/G1 stage. A549 cells were treated with nocodazole 24 h posttransfection and cell cycle distributions were detctected 20 h later. 2N, cells having diploid DNA content; 4N, cells having tetraploid DNA content.