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. 2008 Aug 13;36(16):5391–5404. doi: 10.1093/nar/gkn522

Figure 4.

Figure 4.

miR-16 and miR-424 regulated a set of cell cycle genes. Western blot analysis of CCND3 protein(A), CCNE1(C) and CDK6(E) in A549 cells transfected with dsRNA indicated above the lanes. Antibody against β-actin was used as a loading control. Luciferase assays indicated that miR16 family downregulated the expression of CCND3(B), CCNE1(D) and CDK6(F) directly. pcDNA3.0 control plasmid, miR-16 expressing plasmid and miR-424 expressing plasmid were cotransfected with luciferase reporter vector containing wild-type 3′-UTR of CCND3/CCNE1/CDK6 and their own different mutants of 3′-UTR, respectively. For all histograms, mean values are shown and error bars represent SD from three independent experiments.