Table 2.
mRNAs upregulated in Drosha depleted HepG2 cells compared with control transfected cells and normal HepG2 cells
| Gene name | Ratio of shDrosha /no shRNA | Ratio of shDrosha /control shRNA | Function |
|---|---|---|---|
| SPINK5L3 | 36.15 | 41.79 | |
| SERPINE1 | 11.18 | 13.26 | Blood coagulation, fibrinolysis, regulation of angiogenesis |
| CCND1 | 8.6 | 2.78 | Cell cycle, cell division, fat cell differentiation |
| F3 | 8.04 | 5.53 | Blood coagulation, immune response |
| GADD45A | 7.56 | 3.4 | DNA repair, apoptosis, cell cycle |
| DUSP5 | 6.09 | 3.58 | Protein amino acid dephosphorylation |
| FN1 | 5.74 | 2.43 | Acute-phase response, cell adhesion, cell migration, metabolic process, response to wounding |
| STS-1 | 5.53 | 3.63 | |
| MFAP5 | 5.27 | 12.71 | |
| SAT | 4.91 | 3.92 | Metabolic process |
| SLC19A2 | 4.69 | 3.02 | Sensory perception of sound, thiamin transport |
| ODC1 | 4.69 | 3.12 | Kidney development, polyamine biosynthetic process, cell proliferation |
| ANGPTL4 | 4.43 | 3.64 | Angiogenesis, cell differentiation, development, apoptosis, lipoprotein lipase activity, response to hypoxia |
| MATN2 | 4.35 | 8.4 | Biological process |
| ADM | 4.1 | 4.32 | Cell–cell signaling, circulation, female pregnancy, heart development, cell proliferation |
| NRG1 | 4.09 | 7.04 | Blood pressure regulation, cell differentiation, development, glucose transport, myelination, of transcription |
| CYR61 | 4.03 | 5.53 | Anatomical structure morphogenesis, cell adhesion, cell proliferation, chemotaxis, |
| DKK1 | 3.81 | 2.93 | Wnt receptor signaling pathway, embryonic limb morphogenesis, multicellular organismal development |
| ETS1 | 3.71 | 2.12 | Immune response, cell proliferation, erythrocyte differentiation, regulation of transcription |
| CTPS | 3.69 | 2.93 | Biosynthetic process, metabolic process, response to drug |
| RAPGEF2 | 3.69 | 5.12 | MAPKKK cascade, cAMP-mediated signaling, regulation of small GTPase-mediated signal transduction |
| SLC7A11 | 3.65 | 4.04 | Transport, protein complex assembly |
| CPA4 | 3.47 | 3.71 | Histone acetylation, proteolysis |
| ABHD5 | 3.28 | 3.44 | Lipid metabolic process, proteolysis |
| ENO2 | 3.14 | 6.48 | Glycolysis |
| RND3 | 3.09 | 3.32 | Actin cytoskeleton organization and biogenesis, cell adhesion, small GTPase-mediated signal transduction |
| BMP6 | 3.02 | 4.31 | BMP signaling pathway, development, inflammatory response, osteoblast differentiation, aldosterone biosynthetic process |
| CD44 | 3 | 2.1 | Cell adhesion |
| PPP1R3F | 2.99 | 4.41 | |
| Q9P2H4 | 2.81 | 2.63 | |
| SLPI | 2.75 | 3.45 | Serine-type endopeptidase inhibitor activity |
| GAD1 | 2.69 | 3.26 | Carboxylic acid metabolic process, neurotransmitter biosynthetic process, protein-pyridoxal-5-phosphate linkage, synaptic transmission |
| IGFBP3 | 2.61 | 18.36 | Signal transduction, apoptosis, myoblast differentiation, protein amino acid phosphorylation, cell growth |
| Q7Z2U1 | 2.61 | 2.39 | |
| HDHD1A | 2.51 | 2.13 | Metabolic process |
| DNAJC1 | 2.44 | 2.24 | Proteolysis, protein folding, protein secretion |
| OSMR | 2.36 | 2.4 | Cell proliferation, cell surface receptor linked signal transduction |
| TMEM50A | 2.3 | 3.07 | |
| C21orf34 | 2.26 | 3.29 | |
| ATF3 | 2.21 | 2.08 | Regulation of transcription, DNA-dependent |
| KRT17 | 2.13 | 3.37 | Biological process, epidermis development |
| ABCD3 | 2.12 | 2.78 | Peroxisome organization and biogenesis, transport |
| LIMA1 | 2.11 | 3.33 | Actin filament bundle formation, actin filament depolymerization, ruffle organization and biogenesis |
| C1orf181 | 2.09 | 2.29 | |
| CDKN1A | 2.09 | 5.87 | Cell cycle arrest, apoptosis, cell proliferation, response to DNA damage stimulus, response to UV |