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Accumulation of plastid RNAs in ZmWhy1 mutants. Total seedling leaf RNA (5 μg) was analyzed by RNA gel blot hybridization using probes specific for the RNAs indicated at bottom. The rps12 probe was a cDNA probe containing exons 1 and 2. The leaf pigmentation conditioned by each mutant allele is indicated: iv: ivory; pg: pale green. The methylene blue-stained blots are shown below, with rRNAs marked. Additional RNAs that were analyzed analogously are shown in Supplementary Figure 3A.
