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The stimulatory effect of p12 can be localized to a small peptide, spanning the region that binds BLM. (A) A total of 0.85 nM BLM was incubated at 37°C for 5 min alone (lanes 1–9), or with 20 nM p1271–100 (a peptide that shows no binding to BLM; lanes 10–18), full length p12 (lanes 19–27) or p1230–60 (a peptide that spans the binding region to BLM; lanes 28–36). Reactions were run as in Figure 4B. (B) Quantification of data from (A).
