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. 2008 Sep 22;3(9):e3258. doi: 10.1371/journal.pone.0003258

Figure 2. Characterization of PCMT plasmid constructs.

Figure 2

Panel A) Plasmids were transfected into endothelial cells and 48 h later methyltransferase activity was assayed using a methanol diffusion assay in the presence of saturating concentrations of the methyl donor AdoMet and ovalbumin as a methyl accepting protein. Results are given as the mean of three experiments. Error bars indicate standard deviation; (*) refer to statistically significant differences (p<0.05), as evaluated by t-test. Panel B) Cells transfected were then processed for immunoblotting with PCMT antibody. The immunoblot was reprobed for actin as a loading control. The sample “PCMT” is authentic human recombinant enzyme as a positive control. PAEc = PAE transfected with void plasmid.