Agarose gel electrophoresis of the CCATCGCTACC constructs. Lanes 1 & 2: pMS2 DNA before and after digestion with EcoRV. Lanes 3, 4, 5, and 6 show pMS2 constructs containing C8-AAF-dG, C8-AF-dG, C8-AP-dG, and dG, respectively, after enzymatic removal of the scaffold. Lanes 7 & 8 represent, before and after the removal of the scaffold, respectively, of a “mock” ligation mixture, which did not contain any 11-mer.