Table 1.

Mutational specificity of AAF, AF, and AP adducts in CCATCGCTACC sequence in simian kidney (COS-7) cells^a

Construct	Total colonies screened	G*→ G	C	A	T	Total targeted mutations	Semi-targeted mutations
CGC	698	695 (99.6)b	0	0	0	0 (0)	3c (0.4)
CGAPC	660	639 (96.8)	3 (0.4)	4 (0.6)	9 (1.3)	16 (2.4)	5d (0.7)
CGAFC	740	730 (98.6)	2 (0.3)	0	6 (0.8)	8 (1.1)	2e (0.3)
CGAAFC	590	572 (96.9)	2 (0.3)	4f (0.3)	6 (1.0)	12 (2.0)	6g (1.0)

^aData from several transformations have been combined.

^bThe number in parenthesis shows the frequency in %.

^cA T→G two bases 5′, an A→C three bases 3′, and a C→T five bases 3′ to G; i.e., CCATCGCTACC was changed to CCAGCGCTACC, CCATCGCTCCC, and CCATCGCTACT.

^dA C→A four bases 5′, a T→G two bases 5′, two C→T one base 5′, and an A→C three bases 3′ to G^AP; i.e., CCATCG^APCTACC was changed to CAATCGCTACC, CCAGCGCTACC, CCATTGCTACC, and CCATCGCTCCC.

^eA C→T and a C→A one base 5′ to G^AF; i.e., CCATCG^AFCTACC was changed to CCATTGCTACC and CCATAGCTACC.

^fTwo of these included CG^AAF→TA double mutations.

^gSix A deletions three bases 3′ to G^AAF; i.e., CCATCG^AAFCTACC was changed to CCATCGCT_CC.