. Author manuscript; available in PMC: 2008 Nov 1.

Published in final edited form as: Chem Res Toxicol. 2007 Oct 2;20(11):1658–1664. doi: 10.1021/tx700131e

Table 2.

Mutational specificity of C8-AP-dG in CGCGCG sequence in simian kidney (COS-7) cells

Expt. #	Construct	Total colonies screened	G*→ G	C	A	T	Total targeted mutations	Semi-targeted mutations
1	CGC	168	168 (100)^a	0	0	0	0 (0)	0
2	CGC	119	118 (99.2)	0	0	0	0 (0)	1^b (0.8)
Total	CGC	287	286	0	0	0	0 (0)	1 (0.4)
1	CG^APC	139	128 (92.1)	1 (0.7)	2 (1.4)	8 (5.8)	11 (7.9)	0
2	CG^APC	161	145 (90.1)	2 (1.2)	2 (1.2)	10^c (6.2)	14 (8.7)	2^d (1.2)
Total	CG^APC	300	273 (91.0)	3 (1.0)	4 (1.3)	18 (6.0)	25 (8.3)	2 (0.7)

The number in parenthesis shows the frequency in %.

G deletion at the 3′ ligation site; i.e., CGCGCG was changed to CGCGC_.

Two of these G^AP→T transversions also accompanied an adjacent 3′C deletion; i.e., CGCG^APCG was changed to CGCT_G.

One 5′ C→A adjacent to the adducted G and one +A 5′ to the adducted G; i.e., CGCG^APCG was changed to CGAGCG and CGCaGCG (where the inserted base was shown as an underlined lower case base).