Figure 5. γCOP mutants are defective in Pio secretion.

Pio levels are diminished in γCOP¹⁰ mutants. Secretion of Pio into the lumen of the developing embryonic tracheae in control y w embryos (A, A' blow up). In the hypomorphic mutation γCOP^kg06383 there is only a mild effect on Pio secretion (B, B' blow up of homozygous γCOP^kg06383 mutant embryo of (B)). Only low levels of Pio staining are detectable in the lumen of γCOP¹⁰ mutant embryos (C, C' blow up of homozygous P{neo⁺ FRT}82B sr¹ e^s γCOP¹⁰ mutant embryo of (C)); Secretion of Pio into the tracheal lumen is restored by γCOP expression from the Ω35 rescue construct (D', blow up of homozygous Ω35-17 sr¹ e^s γCOP¹⁰ embryo shown in (D)). In the hypomorphic mutation γCOP^kg06383 there is only a mild effect on Pio secretion (B, B' blow up of homozygous γCOP^kg06383 mutant embryo of (B)). Note that the gain in (C) was increased compared to (A, B, D) in order to show Pio signals (compare background yolk signals)).