Figure 3.

Depletion of CENP-V by RNA interference causes broadening of the primary constriction without affecting heterochromatin levels. (A) Levels of endogenous CENP-V decrease 48 h after transfection with a specific siRNA oligo. Levels of H3K9me3 are not affected when CENP-V is depleted. (B) At 48 h after transfection with control siRNA, chromosomes from cells show a prominent primary constriction. (C) At the same time point, cells transfected with a specific CENP-V siRNA show abnormal centromeric structure. Insets show higher magnification views of chromosomes with ACA, red. (D, E) Quantification of the distance between ACA spots (D) and the width of primary constriction of chromosomes (E). Both values are increased when CENP-V is depleted. Bar, 5 μm.