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. 2008 Sep 4;27(19):2510–2522. doi: 10.1038/emboj.2008.175

Figure 4.

Figure 4

CENP-V depletion by RNAi affects the centromeric localization of chromosomal passenger proteins and Sgo1 but not SMC2. (A) At 48 h after transfection with control siRNA, HeLa cells show normal condensin and INCENP localization. (A′) At same time point, cells transfected with a specific-CENP-V siRNA show normal condensin localization but INCENP staining is decreased and appears delocalized to chromosome arms. (B, B′) Sgo1 and Aurora B localize to centromeres in cells exposed to a control siRNA (B), whereas the staining is reduced or absent in cells exposed to a specific CENP-V siRNA (B′′). Insets show higher magnification views of chromosomes. (C) Aurora B fluorescence intensity levels at centromeres (normalized relative to ACA staining for the same centromeres) are significantly decreased when CENP-V is depleted from cells. Average values are indicated for each condition. (D) Levels of CPC proteins Aurora B and INCENP decrease when cells are transfected with a CENP-V-specific siRNA. (E) Sgo1 fluorescence intensity levels at centromeres (normalized relative to ACA staining for the same centromeres) are significantly decreased when CENP-V is depleted from cells. Average values are indicated for each condition. Bar, 5 μm.