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. 2008 Sep 4;27(19):2510–2522. doi: 10.1038/emboj.2008.175

Figure 7.

Figure 7

Depletion of CENP-V by RNA interference affects the distribution of pericentromeric heterochromatin. (A, A′) Distribution of heterochromatin stained with anti-H3K9me3 in nucleoplasmic foci in HeLa cells at time point zero (A) and at 48 h after transfection with a control siRNA (A′). (A′′) At 48 h after transfection with a specific CENP-V siRNA, the distribution of H3K9me3 was more diffuse in the nucleoplasm. (B) Quantification of the distribution of H3K9me3 in the nucleoplasm of cells depleted of CENP-V. (C) Dendrogram based on image similarity of the H3K9me3 distribution between time point zero (blue), 48 h after transfection with a CENP-V-specific siRNA (green) and 48 h transfection with a control siRNA (red). (D) Immunoblots reveal that the levels of the two characteristic chromatin modifications H3K27me3 and H3K4me2 are not affected when CENP-V is depleted at 48 and 60 h after transfection with specific siRNA. (E) Levels of these modifications also do not change when either CENP-V–GFP or the putative catalytic mutants are overexpressed. Bar, 5 μm.