. Author manuscript; available in PMC: 2008 Sep 9.

Published in final edited form as: J Am Chem Soc. 2006 Mar 8;128(9):3067–3074. doi: 10.1021/ja058093d

Table 3.

Kinetic parameters of [KS3][AT3]-catalyzed chain elongation for ACP2, 3, and 4 with C-terminal linkers. In the ACP2(2) case where condensation was too slow to determine k_cat, k_cat/K_m values were derived from the highest [ACP] (200 uM) time course data assuming K_m,ACP2(2) ≫ 200 uM.

	ACP2(2)	ACP3(0)	ACP3(2)	ACP4(4)
K_m (μM)	N/A	16	32	60
k_cat (1/min)	N/A	0.18	0.16	0.08
Relative k_cat ^#	N/A	100%	88%	45%
k_cat/K_m (1/min-μM) × 1000	0.031	11.0	4.9	1.4
Relative k_cat/K_m ^*	0.2%	100%	45%	12%

(k_cat)_x / (k_cat)_ACP3

(k_cat /K_m)_x / (k_cat /K_m)_ACP3