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Fluorescence micrographs of 12-d corneal epithelial cells (CE), 8-d skin epithelial cells (SE), 12-d corneal fibroblasts (CF), and 8-d skin fibroblasts (SF) exposed to UV irradiation for 2, 3, and 4 min and examined for DNA breaks by ISEL 4 h later. The 4-min exposure induced strong nuclear labeling for DNA breaks in all the cell types tested, except for corneal epithelial cells, in which only occasional fragmented nuclei showed strong labeling (arrows). Bar, 25 μm.
