Table 2.

T-cell infusions

Patient	Infusion 1, cells/m2*	Infusion 2, cells/m2†	Infusion 3, cells/m2‡	Fresh versus thawed cells	Time from apheresis to target cell number, d	No. of stimulation cycles§
A	108	109	3.3 × 109	Fresh	130	7
B	108	109	2 × 109	Fresh	129+	7
C					Expansion failed	5
D	108	4 × 108		Thawed	159+	7
E					Expansion failed	5
F	108	109	3.3 × 109	Thawed	96	6
G	108	109	3.3 × 109	Thawed	90	5
H	108	109	3.3 × 109	Thawed	81	5
I	108	109	2 × 109‖	Thawed	104	8

For patients A through E, T cells were selected and expanded by limiting dilution. For patients F through I, T cells were expanded in bulk culture. Patients C and E did not receive T-cell infusions. Patients B and D received infusions but did not reach the target cell dose.

^*Target dose was 10⁸ cells/m².

^†Target dose was 10⁹ cells/m².

^‡Target dose was 3.3 × 10⁹ cells/m².

^§Defined as the number of times cells were stimulated with OKT3 in the presence of irradiated feeder PBMCs and LCL (repeated every 14 days, first cycle at day 14).

^‖The target number of cells was generated for this patient, but nearly half of the last cell dose was lost during a quality control assay before infusion.