Table 3.

Principal order parameters and revised average orientations with respect to the bilayer membrane normal when using non-zero η parameters from Table 1 for Trp 9, 11, 13 and 15 indole rings of gA channels^a.

Trp	ρ1	ρ2	Szz	Minimum rmsd (kHz)
9	37.0°	23.0°	0.86 ± 0.03	1.14
11	46.0°	23.5°	0.86 ± 0.04	0.66
13	46.5°	27.0°	0.93 ± 0.03	1.87
15	50.5°	30.5°	0.91 ± 0.03	2.00

^aThe angles ρ₁ and ρ₂ (Figure 1) were searched in 0.5° increments, while S_zz was searched in increments of 0.006. The rmsd values correspond to the global minima (comparing observed and calculated quadrupolar splittings). The listed tolerance for S_zz represents the range of S_zz yielding rmsd less than 3.0 kHz.