Figure 3. Representative graphs of alamethicin-induced calcein leakage.
Fluorescence was normalized by the fluorescence level after detergent lysis (0.6 % Triton X 100). The arrows indicate the instant at which the peptide or detergent was added. The concentration of peptide is represented as a molar ratio (alamethicin to polymer or lipid). (A) Alamethicin induces 100% leakage from 200 nm PC vesicles. (B) Alamethicin induces partial calcein leakage from 200 nm OE7 vesicles. (C) Alamethicin does not induce calcein leakage from 200 nm OB18 vesicles.