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. Author manuscript; available in PMC: 2008 Sep 9.
Published in final edited form as: Biochemistry. 2005 Dec 20;44(50):16612–16623. doi: 10.1021/bi0511484

Figure 3.

Figure 3

TTR tetramer reassembly can be measured over a wide concentration range using the environment-sensitive fluorescence exhibited by 1 upon binding to TTR. A) Structure of small molecule 1 with binding constants, as determined by isothermal titration calorimetry. B) Concentration dependent reassembly time courses of wild type TTR homotetramers over a range of protein concentration (0.72 – 36 µM; monomer) monitored by the binding and fluorescence of 1. 0.72 µM – black, 1.08 µM – brown, 1.44 µM – pink, 4.32 µM – orange, 7.2 µM – green, 14.4 µM – blue, 21.6 µM – red, 36 µM – yellow. The inset reveals the complete reassembly time course of 1.08 µM TTR (monomer).

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