. Author manuscript; available in PMC: 2008 Sep 9.

Published in final edited form as: Biochemistry. 2005 Dec 20;44(50):16612–16623. doi: 10.1021/bi0511484

Table 1.

Rate constants and fitting parameters for the best fits of the TTR concentration dependent reassembly kinetics (0.72 – 36 µM, monomer) to mechanism C (A), mechanism D (B), and mechanism E (C), demonstrating that the best fits of the experimental data are obtained from mechanism E, the MDRT pathway.

A Fitting Parameters for Mechanism C
Sum of Squares	k_MD′	k_D′M	k_D′D	k_DD′	k_DT′	k_T′D	k_T′T

0.867915	8.25E+06	1.38E+01	1.57E+02	3.20E+00	1.89E+06	2.11E+01	3.65E-01
0.869983	6.44E+06	1.01E+04	3.91E+04	1.16E+00	1.56E+06	1.62E+01	3.48E-01
0.877087	3.09E+06	2.61E-01	3.37E-01	2.18E+01	1.64E+08	1.19E-264	3.88E+04
B Fitting Parameters for Mechanism D
Sum of Squares	k_MD1	k_D1M	k_MD2	k_D2M	k_D1T	k_D2T

0.359854	6.80E+04	5.08E-02	5.58E+04	1.31E-01	3.01E+05	2.99E-05
0.365402	5.01E+04	4.12E-02	5.01E+04	4.12E-02	3.55E+05	1.61E+00
0.435204	2.19E+04	3.23E-02	1.43E+04	1.10E-01	1.52E+06	1.34E-07
C Fitting Parameters for Mechanism E
Sum of Squares	k_MD	k_DM	k_DR	k_RD	k_RT	K_Dimer	K_Trimer

0.077077	3.28E+05	8.62E+00	7.19E+05	5.67E-03	7.85E+04	2.63E-05	7.89E-09
0.077422	2.57E+06	7.99E+01	7.85E+05	5.66E-03	7.87E+04	3.11E-05	7.22E-09
0.0780296	1.96E+07	5.88E+02	7.53E+05	5.49E-03	7.89E+04	3.00E-05	7.29E-09