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Inhibition of GTPγS-stimulated secretion by (a) N17-Rac2 and (b) N17-Cdc42/G25K. In this experiment the cells were allowed to run down for 7 min before stimulation by Ca2+ (pCa5) and GTPγS (100 μM). Note that the N17-Rac2 preparation is contaminated with about 33% of GST. Results are expressed as means ± SEM (n = 4). •, Cells stimulated with Ca2+ (pCa5) and GTPγS (100 μM); ○, unstimulated cells. Similar results have been obtained on three separate occasions.
