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Leakage of Rac and Cdc42 from mast cells after permeabilization by SL-O. The permeabilized cells were sampled at the times indicated and rapidly sedimented, and the proteins present in the supernatants were separated on 12% SDS-polyacrylamide gels. After transfer to nitrocellulose membranes, the proteins were detected by probing with specific antibodies followed by ECL.
