Figure 3.
Increased TopoIV decreases sister cohesion time. (A) Snapshot analysis in dnaCts TopoIV+ cells containing either an “empty” plasmid (pBAD24) or a plasmid with basal low-level expression of TopoIV (pWX35). The main plot shows the increase in cells containing two ori1 foci in a single experiment carried out over 120 min at 30°C from replication initiation. The inset shows three experiments carried out for a shorter time, with the mean values shown as thick lines and standard errors as vertical bars. (B) Time-lapse analysis of ori1 segregation in DnaC+ cells from replisome appearance at initiation.