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Synthesis of Leucine Enkephalin via α-Nsmoc Amino Acidsa
| Concentration (%) of piperidine in DMF | deblocking time (min) | crude purity by HPLC analysis (%) |
|---|---|---|
| 2 | 1 | 85.9 |
| 5 | 20 sec | 87.7 |
| 5 | 1 | 96.6 |
| 5 | 3 | 94.2 |
| 5 | 5 | 97.0 |
| 20 | 20 sec | 100 |
Three eqv. of the amino acid with 3 eqv. of N-HBTU and 6 eqv. of DIEA and a coupling time of 30 min.
