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. 2008 Sep 9;105(38):14436–14441. doi: 10.1073/pnas.0805163105

Fig. 1.

Fig. 1.

Actin binding by the C terminus of talin is pH-dependent. (A) Actin cosedimentation assays using 10 μg of F-actin and 5 μg of the indicated segments of talin were incubated for 60 min at pH 6.5 and 7.5 and centrifuged to sediment F-actin. Proteins in the pellet and the supernatant were separated by SDS/PAGE and stained with Coomassie. (B) Relative abundance of talin domains cosedimenting with actin was determined by NIH Image software and is expressed as mean ± SEM of four to seven separate assays. (C) Talin–actin binding at pH 6.5 and 7.5 determined with 1 μM of the USH-I/LWEQ domain and the indicated concentrations of F-actin.