Fig. 3.

Mutation of His-2418 modifies pH-dependent structural properties of talin USH-I/LWEQ. (A) Superimposition of the average structures obtained from the last 5 ns of CpHMD for WT (blue) and H2418F mutant (red). The backbone rmsd values obtained between WT at pH 6.0 and mutant at pH 8.0 is 4.8 Å, whereas the rmsd between WT at pH 8.0 and mutant at pH 6.0 is 3.2 Å. (B) 2D 15N-HSQC spectra of WT (blue) and H2418F mutant (red) USH-I/LWEQ talin at pH 6.0 (Upper) and 8.0 (Lower).