Fig. 1.
In vitro and in vivo cytokine responses to RSV infection are lost in the absence of MAVS. Adult lung fibroblasts (A), bone marrow-derived macrophages (BMDM; B and C), and cDCs (D and E) from WT+/+ and Mavs−/− mice were infected with SeV or RSV for 24 h before culture supernatants were analyzed for IFN-β or -α by ELISA. Data are represented as mean ± SD. (F) Mice of the indicated genotypes were intranasally infected with RSV (107 pfu) for 24 h, and then BALF was harvested for cytokine measurements by ELISA (for IFN-α, IFN-β, and IL-1β) or Cytometric Bead Array (for IL-6, TNF-α, and MCP-1) (*, P < 0.001; **, P < 0.01; ***, P < 0.05; ANOVA, Tukey's test) (n = 4 per group). n.d., not detected. Data are represented as mean ± SEM.