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. Author manuscript; available in PMC: 2008 Sep 9.
Published in final edited form as: J Am Chem Soc. 2006 Dec 27;128(51):16684–16691. doi: 10.1021/ja0661414

Figure 1.

Figure 1

Conformationally assisted NCL for chemical synthesis of Grx3 combined with selective oxidation and alkylation. For NCL the thioester-peptide Grx3(1-37)-MPAL (containing either Cys (Y=S) or Sec (Y=Se), at positions 11 and/or 14), mixed with the Cys-peptide Grx3(C38-82). After purification, the ligated product was oxidized to form the disulfide bond (or selenenylsulfide or diselenide bond) to protect them from the alkylation with iodoacetamide. (MPAL = β-mercaptoproparionyl-Leu).