Table 1.

Redox potential and kinetic parameters obtained from direct protein-protein equilibria between reduced Trx and oxidized Grx3 analogs. The second-order rate constants (k₁ and k₋₁) as well as the equilibrium constant, K_eq were calculated by fitting the kinetic data. Redox potential of Grx3 analogs was calculated from K_eq.

Entry	Protein	E0(mV)	Keq.	k1(M−1s−1)	k−1(M−1s−1)
1	sGrxS	−194 ±5	424 ± 170	424 ± 17	1.0 ± 0.4
2	Grx3(C11U)	−260 ±3	2.20 ± 0.52	291 ± 36	132 ± 27
3	Grx3(C14U)	−275 ±5	0.72 ± 0.29	81 ± 17	113 ± 40
4	Grx3(C11U-C14U)	−309 ± 3	0.05 ± 0.01	467 ± 81	9140 ± 2040
5	sGrxS	−197a
6	wt-Grx3	−198b
7	wt-Grx1	−233b
8	wt-Trx1	−270b

^aRedox potential of sGrx3 was confirmed by the wt-Grx1/sGrx3 equilibria; using end-point analysis after reaching equilibrium.

^bData taken from ref. 7.