Figure 7. Enhanced phagocytosis mediated by HBP depends on CD18 but not TLR signaling, while enhanced phagocytosis induced by HNP1–3 is independent of CD18 and TLR signaling.

(A) Peritoneal macrophages were isolated from neutropenic mice of various strains and treated ex vivo with medium, HBP (1 μg/ml), or HNP1–3 (1 μg/ml) for 24 hours. Thereafter, the phagocytic capacity was assessed using IgG-opsonized S. aureus. For each analysis, 4 independent experiments were performed. *P < 0.05 versus respective control. (B) Comparison of phagocytic capacity of peritoneal macrophages from different strains. Mice were rendered neutropenic and then injected i.p. with either PBS or HBP. For each analysis, 3–5 independent experiments were performed. ^†P < 0.05 versus neutropenic mice of the respective strain.