Table 1.
Primers and Probes Used for Real-Time RT-PCR
cDNA | Sense Primer | Antisense Primer | Probe |
---|---|---|---|
SERCA2 | 5' TCT GTC ATT CGG GAG TGG GG 3' | 5' GCC CAC ACA GCC AAC GAA AG 3' | 5' TGG CCA CTC ATG ACA ACC CG 3' |
ANF | 5' CTT GCG GTG TGT CAC ACA GC 3' | 5' GGG AGA GGT AAG GCC TCA CT 3' | 5' CCT CCT GGA GCT GCA GCT TC 3' |
αMHC | 5' ACA GAG TGC TTC GTG CCT GAT 3' | 5' CGA ATT TCG GAG GGT TCT GC 3' | 5' ACA GTC ACC GTC TTG CCG TTT TCA GT 3' |
βMHC | 5' GCT ACC CAA CCC TAA GGA TGC 3' | 5' TCT GCC TAA GGT GCT GTT TCA 3' | 5' TGT GAA GCC CTG AGA CCT GGA GCC 3' |
Probes were labeled at the 5’ end with 6-FAM and at the 3’ end with BHQ-1.