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The ste9 mutants cannot arrest in the G1-phase upon nitrogen starvation. (A) Changes in cell number (open symbols) and percent of septated cells (filled symbols) of cells cultured in EMM2−N. Wild-type (L972, circle) and Δste9 (KJ100–8C, square) strains were used. (B) Wild-type (L972, left) or Δste9 (KJ100–8C, center) strains were cultured in EMM2−N for the duration indicated and analyzed by flow cytometry. The Δrum1 (KJ153–1A, right) is a control for the G1 arrest-defective strain.
