Figure 3.

Synthetic lethality between ste9 and wee1 mutations. (A) Viability for wee1–50 Δcig2 (KJ109–5A, open square), Δste9 wee1–50 Δcig2 (KJ111–1A, filled circle), and Δste9 wee1–50 Δcig2 cells in the presence of HU (open triangle). Log-phase cultures at 23°C were shifted to 35°C at 0 h. HU was added after 2 h of incubation at a final concentration of 12 mM. (B) DNA content of wee1–50 Δcig2 (left) and Δste9 wee1–50 Δcig2 (right) at the restrictive temperature. Cells were shifted to 35°C at 0 h.