Table 3.

Real-time PCR validation of microarray results.

					qPCR fold change		Ratio (T32/S70)
TIGR TC	Annotation	Time (h)	M. truncatula line	Microarray fold change	Mean	SE	Array	qPCR
TC105342	MATE	12	T32	39.8	44.0	7.8	0.8	0.7
			S70	51.3	67.4	6.4
TC95697	F-box protein	12	T32	5.0	13.7	4.0	3.6	4.4
			S70	1.4	3.1	0.2
TC103586	ZPT-2	12	T32	2.9	2.4	0.2	0.8	0.4
			S70	3.5	6.1	0.9
TC111698	COBRA-like gene	12	T32	16.3	24.7	0.6	0.9	0.5
			S70	18.8	53.7	16.4
TC93997	PR-protein PR-4	12	T32	2.6	2.5	0.3	0.5	0.5
			S70	5.0	5.0	1.1
TC100486	Xyloglucan endotransglycosylase	12	T32	2.3	2.2	0.1	1.8	1.6
			S70	1.3	1.4	0.3
TC96658	Xyloglucan endotransglycosylase	48	T32	2.9	3.7	1.0	0.3	0.1
			S70	10.7	38.3	5.6
TC102211	GAST-1	48	T32	2.1	7.2	0.4	1.6	0.3
			S70	1.3	25.8	5.7
TC111920	Polygalacturonase PG1 putative	48	T32	2.8	2.5	0.2	0.8	0.6
			S70	3.7	4.0	0.5
TC103771	Ethylene up- regulated gene ER66	48	T32	1.2	1.3	0.2	0.5	0.3
			S70	2.3	4.6	0.8
TC95154	Peroxidase	48	T32	3.9	1.2	0.1	1.3	0.4
			S70	3.0	2.9	0.1
TC100155	Lipoxygenase	48	T32	0.5	0.4	0.0	0.7	0.6
			S70	0.7	0.6	0.1