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. 2008 Sep 12;283(37):25356–25363. doi: 10.1074/jbc.M802667200

FIGURE 4.

FIGURE 4.

Rereplication-induced Cdt1 degradation is independent of geminin sequestration. A, HCT116 cells were infected with WT Cdt1-V5, PIPm Cdt1-V5, or control adenovirus at an m.o.i of 40 and with WT HA-Cdt1 or ΔC HA-Cdt1 at a m.o.i of 100 (+) or 300 (++) for 24 h followed by immunoblot analysis using the indicated antibodies. B, HCT116 cells were infected with control, WT HA-Cdt1, or ΔC HA-Cdt1 recombinant adenovirus at an m.o.i of 150. 24 h after infection, cells were harvested and probed for ectopic Cdt1 with anti-HA antibody or for rereplication by flow cytometry. C, HCT116 cells were infected as in B for 24 h. Cell lysates were probed for ectopic Cdt1 with anti-HA antibody (top panel) or were subjected to immunoprecipitation with anti-HA antibody and probed for endogenous geminin (bottom panel).