FIGURE 1.

Effects of single-amino acid substitutions in MccJ25 on production, maturation, export, and stability of MccJ25. Data are presented for 380 single-amino acid substitution derivatives of MccJ25, comprising all possible substitutions at positions 1-7 and 9-21. The sequence of MccJ25 is shown at the bottom. The heights of bars indicate the percentage of tested amino acid substitutions that do not prevent production, maturation, export, and stability of MccJ25. Letters within bars list amino acid substitutions that do not prevent production, maturation, and stability of MccJ25. (Position 8 (gray bar) was not subjected to saturation mutagenesis in this work; see “Results.”)