FIGURE 8.

Effects of forced expression of ERp16 or ERp16-CS on apoptosis induced by tunicamycin or dithiothreitol. HeLa cells stably expressing wild-type ERp16 (WT) or ERp16-CS (CS) were treated with 3 μm tunicamycin (Tm) for 54 h or with 6 mm dithiothreitol (DTT) for 12 h, after which they were subjected to flow cytometric analysis of the number of apoptotic cells (A), to assay of caspase-3-like activity (B), or to immunoblot analysis (C) as described in the legend to Fig. 7. Data in A and B are means ± S.E. of values from three independent experiments. FAU, fluorescence arbitrary units.