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. 2008 Sep 12;283(37):25606–25616. doi: 10.1074/jbc.M802492200

FIGURE 6.

FIGURE 6.

hnRNP L interacts with CD154 mRNA in a 3′-UTR CARE-dependent manner. a, PTB and hnRNP L were immunoprecipitated from cytosolic and nuclear extracts from activated (PMA/ionomycin, 24 h) T cells. PTB (BB7 MoAb), hnRNP L (4D11), and P3 (irrelevant isotype control) were used. PTB and hnRNP L were coimmunoprecipitated in both extracts, although hnRNP L in PTB immunoprecipitate is not appreciated with this exposure. Immediately below is shown CD154 mRNA in hnRNP L and PTB immunoprecipitates from both cytoplasm and nucleus. No GAPDH, interleukin-2, or γ-interferon mRNA was detectable in either PTB or hnRNP L immunoprecipitates. b, immunoblot of hnRNP L immunoprecipitate from cytoplasmic extracts from HeLa cells transfected with control, CARE, or CD154 3′-UTR containing luciferase vectors or no vector (mock). c, hnRNP L immunoprecipitates contain luciferase mRNA in a 3′-UTR CARE-dependent manner, since no product is seen in extracts transfected with control vector lacking these sequences. Further specificity is shown by inability to detect GAPDH mRNA in immunoprecipitates.