TABLE 1.
Kinetic parameters for AdPLA with different substrates
| Compounda | Vmax (μmol/min/mg pro) | Km (μm) | Vmax/Km |
|---|---|---|---|
| 1-Palmitoyl-2 linoleoyl-PC | 3.23 | 18.6 | 0.17 |
| Dilinoleoyl-PC | 5.43 | 17.5 | 0.31 |
| 1-Palmitoyl-2-linoleoyl-PS | 0.56 | 7.25 | 0.08 |
| 1-Palmitoyl-2-linoleoyl-PE | 0.84 | 5.01 | 0.17 |
| 1-Palmitoyl-2-linoleoyl-PA | 0 | ||
| Phosphatidylinositol | 2.90 | 11.64 | 0.25 |
| 1-Palmitoyl-2-arachidonyl-PC | 2.15 | 8.24 | 0.26 |
a
Recombinant AdPLA was incubated with micelles containing the indicated substrates in 50 mm Tris, pH 8.0 with 2 mm deoxycholate, in the absence of added calcium (n = 3–5). Reactions were monitored by spectrophotometric assay of the change in A234. PS is phosphatidylserine; PA is phosphatidic acid; PE is phosphatidylethanolamine