FIGURE 1.

TRα shuttling is inhibited by treatment with LMB in live monokaryons expressing CRT. A, HeLa cells were transfected with a GFP-TRα expression plasmid and nucleocytoplasmic shuttling was monitored through FRAP (n = 7). White arrowheads indicate photobleached nuclei. Parallel experiments were performed in the presence of LMB (n = 6) to block CRM1-mediated nuclear export and DIC images were taken to delineate cell borders. Fluorescence recovery graphs indicating relative shuttling of GFP-TRα were generated. Black squares indicate relative fluorescence levels within unbleached nuclei and open diamonds represent levels within bleached nuclei. Any apparent change in nucleus morphology is a result of cell movement over the course of the experiment. Error bars, ± 1 S.E. B, as in A using crt^+/+ cell line (n = 10, -LMB; n = 6, +LMB). Bar, 10 μm.