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The CRM1-mediated nuclear export pathway is active in crt-/- cells. An expression plasmid for a CRM1-dependent shuttling control protein (GFP-GST-NES-NLS) was transfected into crt-/- cells and nucleocytoplasmic shuttling was monitored by FRAP. White arrowheads represent bleached nuclei. Bar, 10 μm.
