Table 2.
Glutaredoxin activity in strains overexpressing GRX1 and GRX2
Strain | Wild-type exponential | Wild-type stationary | grx1 grx2 stationary |
---|---|---|---|
pRS426 | 27 ± 4 | 152 ± 12 | 6 ± 0.4 |
mcGRX1 | 107 ± 4 | 486 ± 18 | 115 ± 19 |
pRS424 | 37 ± 7 | 184 ± 50 | 13 ± 5 |
mcGRX2 | 215 ± 11 | 411 ± 55 | 573 ± 114 |
Glutaredoxin activity is given as nanomoles of NADPH oxidized per minute per milligram of protein. Data are the means of triplicate experiments.