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. Author manuscript; available in PMC: 2008 Sep 10.
Published in final edited form as: Biochemistry. 2007 Feb 2;46(8):2233–2238. doi: 10.1021/bi6023397

FIGURE 8.

FIGURE 8

Dependence of oxidation of fibroblast cholesterol on Ca++ and ionomycin. Cultured fibroblasts were dissociated from their flasks (3), washed and resuspended in HBS. Aliquots (containing ~2 μg cholesterol) were suspended in a final volume of 500 μl containing 0 or 1 mM CaCl2, 0 or 1 mM EGTA and 0 or 1 μM ionomycin. The mixtures were incubated for 10 min at 37 °C, 2 IU cholesterol oxidase was added and the incubation continued for 20 min at 37 °C. The oxidation of cholesterol was then determined by HPLC.