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. 1998 May;9(5):1093–1105. doi: 10.1091/mbc.9.5.1093

Figure 6.

Figure 6

The levels of IR β-subunit, IRS-1, p85, ras-GAP, and AFP expression in AFP+, AFP±, and AFP− clones of the McA-RH 7777 rat hepatoma cell line. Total proteins were isolated from cells of the stable AFP− clone 7H10 (lane 1), the unstable AFP− clone H11 (lane 2), the AFP± clone D7 (lane 3), the unstable AFP+ clone A3 (lane 4), the stable AFP+ clones 7A1 (lane 5), and 7G4 (lane 6), separated by SDS-PAGE and Western blotted with antibodies to IR β-subunit (IRβ), IRS-1, p85 regulatory subunit of PIK (p85), ras-GAP, and AFP. Equal protein loading (50 μg/lane) was verified by Coomassie brilliant blue staining of a gel duplicate. Molecular weight markers are indicated.