Figure 8.

Effect of insulin on expression and tyrosine phosphorylation of IR β-subunit in cells of AFP⁺ and AFP⁻ clones of the McA-RH 7777 hepatoma cell line. Cells of the stable AFP⁺ clone 7G3 were incubated at 37°C without insulin (lane 1) or with 100 nM insulin for 20 min (lane 2) or 3 h (lane 3). Cells of the stable AFP⁻ clone 7F3 were incubated at 37°C without insulin (lane 4) or with 100 nM insulin for 20 min (lane 5) or 3 h (lane 6). Proteins from control and insulin-stimulated cells were Western blotted with antibodies to PY or IR β-subunit (IRβ).