Table 2.
Myosin | Vbasal†, s−1 |
Vmax‡, s−1 |
Km§, μM |
|||
---|---|---|---|---|---|---|
Unlabeled | Labeled | Unlabeled | Labeled | Unlabeled | Labeled | |
Muscle | 0.10 ± 0.02 | 0.32 ± 0.13 | 13.5 ± 0.5 | 2.4 ± 0.2 | 13.8 ± 2.3 | 7.2 ± 0.3 |
Dicty | 0.15 ± 0.03 | 0.42 ± 0.05 | 4.9 ± 0.8 | 1.9 ± 0.4 | 12.8 ± 1.8 | 8.8 ± 1.8 |
†In the absence of actin.
‡At saturating actin.
§Actin concentration at which V = 0.5 Vmax.