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RCS1 is specifically ubiquitinated by APC/C-Cdh1 and APC/C-Cdc20 in a D-box-dependent manner. (A) Ubiquitination assay for Plk1 and RCS1 by APC/C-Cdh1 and APC/C-Cdc20 as described in Fig. 1. (B) Schematic representation of RCS1 depicting two D-box sequences. (C) Ubiquitination assay for wild-type and mutant RCS1 by APC/C-Cdh1 as described in Fig. 1.
