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. 2008 Aug 28;105(36):13421–13426. doi: 10.1073/pnas.0801613105

Fig. 2.

Fig. 2.

SIRT1 is a target of microRNA miR-34. (A) Dose-dependent suppression of SIRT1 by miR-34a in HeLa cells. HeLa cells were transfected with 5, 10, or 20 nM miRNA precursors and immunoblotted as above. (B) Quantification by densitometry of effects of premiR-34a on SIRT1 expression (n = 3 ± SD; *, P < 0.05 compared with scrambled mRNA). (C) Dose-dependent increase of SIRT1 by miR-34a in HEK293 cells. HEK293 cells were transfected with 15 or 30 nM antisense miRNA and immunoblotted as above. (D) Quantification by densitometry of effects of knocking down endogenous miR-34a on SIRT1 expression (n = 3 ± SD; *, P < 0.05 compared with scrambled mRNA).