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. 2008 Aug 28;105(36):13439–13444. doi: 10.1073/pnas.0806106105

Fig. 3.

Fig. 3.

Isolation of mutations in the ANTP HD affecting EY protein interaction. (A) One-and-a-half hybrid of Antp-mediated repression of EY activity. Several independent colonies were tested independently. EY, positive control showing the activation of so10-LacZ by EY. EY/ANTP, coexpression of EY and ANTP (GAL4AD-Antp HD) results in so10-LacZ repression. EY/Antp G15 or G19 or G27, capacity of Antp mutants in repressing EY function. None of the mutants lost completely the ability to repress EY activity. (B and C) Gel shift experiments on HB1 (B) or a Hox/Exd/Hth-binding site in the presence of EXD and HTH proteins (C). (B) Upper band indicates the specific complex. (C) Only the ternary complex is shown. (D) GST pulldown experiment between the EY PD (GST-EY-PD) and full-length ANTP mutant proteins (indicated on top of each lane).