Table 1.
Fly phenotypes observed using different UAS-ANTP mutant lines
| Mutant lines | dpp-GAL4 |
OK107-GAL4 |
||
|---|---|---|---|---|
| Antenna to leg | Eye reduction | Pupal lethality, % | Eye reduction/WT (escapers or pupae), % | |
| UAS-Antp | + | +/++ | ∼95 | 75–100 |
| UAS-AntpH5 | + | ++ | <10 | 50–75 |
| UAS-AntpA5 | − | +/− | <10 | 25–50 |
| UAS-AntpK50 | − | − | <10 | 25–50 |
| UAS-AntpA50,51 | − | − | 0 | 10–50 |
| UAS-AntpH53 | − | − | 0 | <10 |
| UAS-AntpG15 | + | + | ∼99 | 75–100 |
| UAS-AntpG19 | + | +/− | ∼99 | 25–50 |
| UAS-AntpA27 | + | ++ | ∼99 | 75–100 |
By expressing WT ANTP in larvae using different GAL4 drivers, distinct phenotypes are distinguished: antenna-to-leg transformation, eye reduction, and pupal lethality (resulting from a headless phenotype). ANTP mutants (A5, K50, A50, 51, H53) affecting DNA binding activity are unable to induce antenna-to-leg transformation and pupal lethality. Most of these lines retain the capacity to inhibit eye development when expressed in the eye disc. By contrast, the ANTP mutant G19, retaining full DNA-binding activity in vitro, induced antenna-to-leg transformation and pupal lethality but exhibited a weaker ability to induce eye reduction. Percentage of eye reduction given was estimated by comparison with a normal WT eye size.